However, because of limits in the draft genome sequence, a considerable number of PhE2F/DPs were still missing annotations. Previous studies identified 12 PhE2F/DP transcription factors from the first version of the Moso bamboo genome database. In plants, E2F/DP binds to the E2FAT (TTTCCCGCC) motif in its target genes, playing a role in promoting transcription. Studies on E2F in Arabidopsis have indicated that plants also have all the core regulators in the E2F signaling pathway, such as cyclin-dependent kinase inhibitors (CKIs), retinoblastoma (RBs), cyclins, and cyclin-dependent kinases (CDKs). In mammals, the E2F signaling pathway is essential for cell growth and cell proliferation. The E2F member might perform distinct roles in controlling the cell fate determination. E2Fa and E2Fb interact with DPa to activate the cell cycle and cell proliferation-associated gene expression through the organization of their leucine zipper dimerization domains. During cell proliferation, the canonical AtE2Fs reportedly play an antagonistic role, because E2Fc is a negative regulator, whereas E2Fa and E2Fb are positive regulators. The E2F/DP family has eight members in Arabidopsis. The DEL group genes are considered to be atypical E2F/DPs and act in monomeric form, and the DEL genes only contain a duplicated DNA-binding domain. The E2F group gene contains four functional domains including an RBR-binding domain, a ‘marked box’ domain, a DNA-binding domain, and a leucine zipper dimerization domain, while the members of the DP group lack the RBR-binding domain, a ‘marked box’ domain compared with E2F. The E2F/DP transcription factors in higher plants are categorized into E2F, DP, and DEL (DP-E2F-like) groups based on their conserved domains. Cell division played critical roles during the winter and early growth periods, while cell elongation was predominant during the late growth period. During Moso bamboo shoot growth, the height increase was created by simultaneous cell division and cell elongation. It is the most important non-timber product used for commercial purposes in East Asia due to its fast growth rate. Moso bamboo ( Phyllostachys edulis) generates nearly 5 billion US dollars annually in economic value. Our findings provide novel insights into the Moso bamboo E2F/DP family and partial experimental evidence for further functional verification of the PheE2F/DPs. The qRT-PCR results showed that the PheE2F/DPs exhibited diverse expression patterns in response to drought and salt treatment and diurnal cycles. A yeast two-hybrid assay and expression analysis based on transcriptome data and in situ hybridization indicated that the PheE2F-PheDP complex played important roles in winter Moso bamboo shoot growth. A regulatory element and coexpression network analysis indicated that E2F/DP regulated the expression of cell cycle-related genes. An estimation of the divergence time of the paralogous gene pairs suggested that the E2F/DP family expansion primarily occurred through a whole-genome duplication event. Here, we identified 23 E2F/DPs in the Moso bamboo genome, including nine E2F genes, six DP genes, eight DEL genes and one gene with a partial E2F domain. However, to date, research into the potential role of the Moso bamboo E2F/DP family has been limited. E2F/DP proteins have been shown to regulate genes implicated in cell cycle control and DNA repair.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |